Isolation and Expression of Human Cytokine Synthesis Inhibitory Factor cDNA Clones: Homology to Epstein-Barr Virus Open Reading Frame BCRFI

We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1991-02, Vol.88 (4), p.1172-1176
Hauptverfasser:	Vieira, P., de Waal-Malefyt, R., M.-N. Dang, Johnson, K. E., Kastelein, R., Fiorention, D. F., de Vries, J. E., M.-G. Roncarolo, Mosmann, T. R., Moore, K. W.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques AMINO ACID SEQUENCE AMINO ACIDS Analysis of the immune response. Humoral and cellular immunity ANIMAL CELLS ANIMALS ANTIGENS B lymphocytes BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOSYNTHESIS CARBOXYLIC ACIDS Cell Line Cell lines Cloning, Molecular Complementary DNA CONNECTIVE TISSUE CELLS COS cells Cytokines DAYS LIVING RADIOISOTOPES DNA DNA - genetics DNA - isolation & purification DNA SEQUENCING DRUGS EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology Fundamental immunology genes Granulocyte-Macrophage Colony-Stimulating Factor - antagonists & inhibitors Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis GROWTH FACTORS Herpesvirus 4, Human - genetics Humans Immunobiology IMMUNOLOGY INHIBITION Interferon-gamma - antagonists & inhibitors Interferon-gamma - biosynthesis Interleukin-10 Interleukins - genetics Interleukins - pharmacology ISOTOPES Leukocytes, Mononuclear - immunology LIGHT NUCLEI LIPOTROPIC FACTORS LYMPHOKINES Lymphokines, interleukins ( function, expression) MAMMALS MAST CELLS Messenger RNA METHIONINE MICE MITOGENS Molecular Sequence Data MOLECULAR STRUCTURE NUCLEI NUCLEIC ACIDS Open Reading Frames ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS Polymerase Chain Reaction PROTEINS RADIOISOTOPES RECOMBINANT DNA Recombinant Proteins - pharmacology Regulatory factors and their cellular receptors RNA RNA, Messenger - genetics RODENTS Sequence Homology, Nucleic Acid SOMATIC CELLS STRUCTURAL CHEMICAL ANALYSIS SULFUR 35 SULFUR ISOTOPES SYNTHESIS T lymphocytes Transfection VERTEBRATES
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Zusammenfassung:	We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BCRFI. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.88.4.1172