Carbohydrate-Dependent Hemolytic Activity of the Conjugate Composed of a C-Type Lectin, CEL-I, and an Amphiphilic α-Helical Peptide, 43-βAla2

A lectin-cationic peptide conjugate, 4 3 -CEL-I, was prepared from an invertebrate C-type lectin, CEL-I, and an amphiphilic α-helical peptide, 4 3 -βAla 2 [Ac-(Leu-Ala-Arg-Leu) 3 -βAla 2 ]. When 4 3 -CEL-I was incubated with rabbit erythrocytes, hemolysis was observed, especially at basic pH. Inhibi...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-01, Vol.63 (7), p.1312-1314
Hauptverfasser:	HATAKEYAMA, Tomomitsu, KAMINE, Tetsuji, KONISHI, Yuko, KUWAHARA, Hiromiki, NIIDOME, Takuro, AOYAGI, Haruhiko
Format:	Artikel
Sprache:	eng
Schlagworte:	amphiphilic α-helical peptide Biological and medical sciences Biotechnology carbohydrate-binding conjugation Fundamental and applied biological sciences. Psychology hemolysis lectin Methods. Procedures. Technologies Protein engineering
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A lectin-cationic peptide conjugate, 4 3 -CEL-I, was prepared from an invertebrate C-type lectin, CEL-I, and an amphiphilic α-helical peptide, 4 3 -βAla 2 [Ac-(Leu-Ala-Arg-Leu) 3 -βAla 2 ]. When 4 3 -CEL-I was incubated with rabbit erythrocytes, hemolysis was observed, especially at basic pH. Inhibition experiment using some carbohydrates suggested that hemolytic activity of 4 3 -CEL-I was caused by the interaction between 4 3 -βAla 2 portion in the conjugate and the lipid bilayer after binding to the carbohydrate chains on the cell surface by the lectin activity of CEL-I.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.63.1312