PAPS‐reductase of Escherichia coli

The DNA of the gene complementing a PAPS‐reductase‐deficient strain of Escherichia coli was sequenced. The N‐terminal amino acid sequence of the purified PAPS‐reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of...

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Veröffentlicht in:	FEBS letters 1990-01, Vol.260 (1), p.6-9
Hauptverfasser:	Krone, Frank A., Westphal, Goetz, Meyer, Helmut E., Schwenn, Jens D.
Format:	Artikel
Sprache:	eng
Schlagworte:	3'-Phospho-adenylylsulfate reductase APS, adenylylsulfate Biological and medical sciences cys H DNA Escherichia coli Fundamental and applied biological sciences. Psychology Gene product Genes. Genome Molecular and cellular biology Molecular genetics PAPS, 3'-phospho-adenylyl-sulfate Phenotypic complementation PT7 expression SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis
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Beschreibung
Zusammenfassung:	The DNA of the gene complementing a PAPS‐reductase‐deficient strain of Escherichia coli was sequenced. The N‐terminal amino acid sequence of the purified PAPS‐reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of M r = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(90)80052-K