Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

The usefulness and limitations of the Becton–Dickinson fluorescence‐activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to pr...

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Veröffentlicht in:Review of scientific instruments 1988-09, Vol.59 (9), p.1928-1932
Hauptverfasser: Muller, Claude P., Krabichler, Gert
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Sprache:eng
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Exact sciences and technology
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Physics
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container_end_page 1932
container_issue 9
container_start_page 1928
container_title Review of scientific instruments
container_volume 59
creator Muller, Claude P.
Krabichler, Gert
description The usefulness and limitations of the Becton–Dickinson fluorescence‐activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.
doi_str_mv 10.1063/1.1140053
format Article
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ispartof Review of scientific instruments, 1988-09, Vol.59 (9), p.1928-1932
issn 0034-6748
1089-7623
language eng
recordid cdi_pascalfrancis_primary_19550748
source AIP Digital Archive
subjects Exact sciences and technology
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Physics
title Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter
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