Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

The usefulness and limitations of the Becton–Dickinson fluorescence‐activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to pr...

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Veröffentlicht in:	Review of scientific instruments 1988-09, Vol.59 (9), p.1928-1932
Hauptverfasser:	Muller, Claude P., Krabichler, Gert
Format:	Artikel
Sprache:	eng
Schlagworte:	Exact sciences and technology Instruments, apparatus, components and techniques common to several branches of physics and astronomy Physics
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	The usefulness and limitations of the Becton–Dickinson fluorescence‐activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.
ISSN:	0034-6748 1089-7623
DOI:	10.1063/1.1140053