Construction and characterization of Escherichia coli disruptants defective in the yaeM gene

Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the no...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-04, Vol.63 (4), p.776-778
Hauptverfasser:	Kuzuyama, T. (Tokyo Univ. (Japan)), Takahashi, S, Seto, H
Format:	Artikel
Sprache:	eng
Schlagworte:	2-C-methyl-D-erythritol 4-phosphate 2-Methyl-D-erythritol 2-Methyl-D-erythritol 4-phosphate Aldose-Ketose Isomerases - biosynthesis Aldose-Ketose Isomerases - genetics Bacteriology Biological and medical sciences biosynthesis Blotting, Southern Catalysis Cloning, Molecular DNA, Fungal - biosynthesis DNA, Fungal - genetics Erythritol - analogs & derivatives Erythritol - metabolism ESCHERICHIA COLI Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology GENE GENES Genes, Bacterial - genetics Genetics Microbiology Multienzyme Complexes - biosynthesis Multienzyme Complexes - genetics Mutation - genetics Mutation - physiology nonmevalonate pathway Oxidoreductases - biosynthesis Oxidoreductases - genetics Pentosephosphates - metabolism Phenotype Sugar Phosphates - metabolism terpenoid TERPENOIDE TERPENOIDOS TERPENOIDS yaeM gene
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Zusammenfassung:	Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.63.776