Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing
A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tu...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2006-12, Vol.44 (12), p.4566-4568 |
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| container_end_page | 4568 |
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| container_issue | 12 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 44 |
| creator | Shi, Ruiru Zhang, Jianyuan Li, Chuanyou Kazumi, Yuko Sugawara, Isamu |
| description | A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates. |
| doi_str_mv | 10.1128/JCM.01916-06 |
| format | Article |
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This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.01916-06</identifier><identifier>PMID: 17035499</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Anti-Bacterial Agents - pharmacology ; Biological and medical sciences ; China ; Chromatography, High Pressure Liquid ; DNA Gyrase - genetics ; DNA Mutational Analysis ; DNA, Bacterial - genetics ; Drug Resistance, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Mutation, Missense ; Mycobacteriology and Aerobic Actinomycetes ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Ofloxacin - pharmacology ; Point Mutation ; Sequence Analysis, DNA ; Tuberculosis - microbiology</subject><ispartof>Journal of Clinical Microbiology, 2006-12, Vol.44 (12), p.4566-4568</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-be0c88b6a4f4cbfd4630d79878aa03db4361601eeefbaedef029b8e6394512f53</citedby><cites>FETCH-LOGICAL-c494t-be0c88b6a4f4cbfd4630d79878aa03db4361601eeefbaedef029b8e6394512f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698392/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698392/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,3175,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18366993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17035499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Ruiru</creatorcontrib><creatorcontrib>Zhang, Jianyuan</creatorcontrib><creatorcontrib>Li, Chuanyou</creatorcontrib><creatorcontrib>Kazumi, Yuko</creatorcontrib><creatorcontrib>Sugawara, Isamu</creatorcontrib><title>Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>China</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA Gyrase - genetics</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Mutation, Missense</subject><subject>Mycobacteriology and Aerobic Actinomycetes</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Ofloxacin - pharmacology</subject><subject>Point Mutation</subject><subject>Sequence Analysis, DNA</subject><subject>Tuberculosis - microbiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxSMEoqVw4wwWEpxIsWPHm1yQVttCi3YpoqzEzZo4k6yrxN7aCZDvyIfCy64onDj532-e32hekjxl9JSxrHjzYbE6paxkMqXyXnLMaFmkUtKv95NjSss8ZYzPjpJHIdxQyoTI84fJEZtRnouyPE5-nvfoW7QaiWvIVdO5H6CNJZ8xmDDA7j6eVpN2FegBvRl7MowVej12LiJk0RlrNHTkMrgOBgyk8a4ni42xQCCQM4xVvbFYk2oi7eTnZDUOMBhnydxCN-1E1sHYNqIWhtHvthem3aSfPIYweiRLczuaOmpGZRhc62G7mQjYmpx9nJNrvB1jA7HscfKggS7gk8N6kqzfnX9ZXKTLq_eXi_ky1aIUQ1oh1UVRSRCN0FVTC8lpPSuLWQFAeV0JLpmkDBGbCrDGhmZlVaDkpchZ1uT8JHm7192OVY-1Rjt46NTWmx78pBwY9e-LNRvVum-KybLgZRYFXh0EvIvmw6B6EzR2HVh0Y1CyyDIuM_5fkJU5E5LSCL7eg9q7EDw2f9wwqnY5UTEn6ndOFJURf_Z3B3fwIRgReHkAIMTpNj5GwYQ7ruBSRixyL_bcJk7su_GoIPTqRvdKCMUyJXK5--35HmrAKWh9FFpfZ5RxGp1RKmf8F5p64E4</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Shi, Ruiru</creator><creator>Zhang, Jianyuan</creator><creator>Li, Chuanyou</creator><creator>Kazumi, Yuko</creator><creator>Sugawara, Isamu</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061201</creationdate><title>Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing</title><author>Shi, Ruiru ; Zhang, Jianyuan ; Li, Chuanyou ; Kazumi, Yuko ; Sugawara, Isamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-be0c88b6a4f4cbfd4630d79878aa03db4361601eeefbaedef029b8e6394512f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>China</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA Gyrase - genetics</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Mutation, Missense</topic><topic>Mycobacteriology and Aerobic Actinomycetes</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Ofloxacin - pharmacology</topic><topic>Point Mutation</topic><topic>Sequence Analysis, DNA</topic><topic>Tuberculosis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Ruiru</creatorcontrib><creatorcontrib>Zhang, Jianyuan</creatorcontrib><creatorcontrib>Li, Chuanyou</creatorcontrib><creatorcontrib>Kazumi, Yuko</creatorcontrib><creatorcontrib>Sugawara, Isamu</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Ruiru</au><au>Zhang, Jianyuan</au><au>Li, Chuanyou</au><au>Kazumi, Yuko</au><au>Sugawara, Isamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>44</volume><issue>12</issue><spage>4566</spage><epage>4568</epage><pages>4566-4568</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. 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| subjects | Anti-Bacterial Agents - pharmacology Biological and medical sciences China Chromatography, High Pressure Liquid DNA Gyrase - genetics DNA Mutational Analysis DNA, Bacterial - genetics Drug Resistance, Bacterial - genetics Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Mutation, Missense Mycobacteriology and Aerobic Actinomycetes Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Ofloxacin - pharmacology Point Mutation Sequence Analysis, DNA Tuberculosis - microbiology |
| title | Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing |
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