Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing

A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tu...

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Veröffentlicht in:	Journal of Clinical Microbiology 2006-12, Vol.44 (12), p.4566-4568
Hauptverfasser:	Shi, Ruiru, Zhang, Jianyuan, Li, Chuanyou, Kazumi, Yuko, Sugawara, Isamu
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Biological and medical sciences China Chromatography, High Pressure Liquid DNA Gyrase - genetics DNA Mutational Analysis DNA, Bacterial - genetics Drug Resistance, Bacterial - genetics Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Mutation, Missense Mycobacteriology and Aerobic Actinomycetes Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Ofloxacin - pharmacology Point Mutation Sequence Analysis, DNA Tuberculosis - microbiology
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Zusammenfassung:	A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.01916-06