Use of Bioluminescent Indicator Acinetobacter Bacterium for Screening and Characterization of Active Antimicrobial Agents

Because of the need for new antimicrobial substances with novel mechanisms of action, we report here the use of an Acinetobacter reporter system for high-throughput screening of active antimicrobial agents. The bioreporter Acinetobacter strain DF4/PUTK2 carrying luciferase genes luxCDABE was chosen...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006-11, Vol.16 (11), p.1706-1712
Hauptverfasser:	Abd-El-Haleem, Desouky A.M. (Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, New Burg-Elarab City, Alexandria, Egypt), E-mail: abdelhaleemm@yahoo.de, Zaki, Sahar A. (Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, New Burg-Elarab City, Alexandria, Egypt)
Format:	Artikel
Sprache:	eng
Schlagworte:	antimicrobial activities BACILLUS CEREUS BACTERIOCINAS BACTERIOCINE BACTERIOCINS BIOASSAYS Biological and medical sciences bioluminescence bioreporter Biotechnology ENSAYO BIOLOGICO Fundamental and applied biological sciences. Psychology TEST BIOLOGIQUE
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Zusammenfassung:	Because of the need for new antimicrobial substances with novel mechanisms of action, we report here the use of an Acinetobacter reporter system for high-throughput screening of active antimicrobial agents. The bioreporter Acinetobacter strain DF4/PUTK2 carrying luciferase genes luxCDABE was chosen because of its ecological importance and it is widespread in nature. This bioreporter is genetically engineered to emit light constitutively that can be measured in real time by luminometry.
ISSN:	1017-7825