Domain Function and Relevant Enzyme Activity of Cycloinulooligosaccharide Fructanotransferase from Paenibacillus polymyxa

Domain Function and Relevant Enzyme Activity of Cycloinulooligosaccharide Fructanotransferase from Paenibacillus polymyxa

Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cycloinulooligosaccharides (cyclofructan, CF) of β-(2→1)-linked D-fructofuranose as well as hydrolysis of cyclofructan. Sequences analysis indicated that CFTase divided into five distinct regions containing three repeated se...

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Veröffentlicht in:Han'gug mi'saengmul saengmyeong gong haghoeji 2006-09, Vol.34 (3), p.278-287
Hauptverfasser: You, D.J. (Osaka University, Osaka, Japan), Park, J.H. (Dongeui University, Busan, Republic of Korea), You, K.O. (Dongeui University, Busan, Republic of Korea), Nam, S.W. (Dongeui University, Busan, Republic of Korea), Kim, K.H. (Dongeui University, Busan, Republic of Korea), Kim, B.W. (Dongeui University, Busan, Republic of Korea), Kwon, H.J. (Dongeui University, Busan, Republic of Korea), E-mail: hjkwon@deu.ac.kr
Format: Artikel
Sprache:kor
Schlagworte:
BACILLUS
cyclofructan
cycloinulooligosaccharide frctanotransferase
deletion mutant
Paenibacillus polymyxa
repeat region
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Zusammenfassung:Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cycloinulooligosaccharides (cyclofructan, CF) of β-(2→1)-linked D-fructofuranose as well as hydrolysis of cyclofructan. Sequences analysis indicated that CFTase divided into five distinct regions containing three repeated sequences (R1, R3, and R4) at the N-terminus and C-terminus. Each domain function was investigated by comparison of wild type CFTase enzyme (CFT148) and deletion mutant proteins (CFT108: R1 and R3 deletion; CFT130:R4 deletion; and CFT88: R1, R3, and R4 deletion) of CFTase.
ISSN:1598-642X