Characterization of Styrene Catabolic Genes of Pseudomonas putida SN1 and Construction of a Recombinant Escherichia coli Containing Styrene Monooxygenase Gene for the Production of (S)-Styrene Oxide

Characterization of Styrene Catabolic Genes of Pseudomonas putida SN1 and Construction of a Recombinant Escherichia coli Containing Styrene Monooxygenase Gene for the Production of (S)-Styrene Oxide

Some Pseudomonas species can grow on styrene as a sole carbon and energy source. From the new isolate Pseudomonas putida SN1, the genes for styrene catabolism were cloned and sequenced. They were composed of four structural genes for styrene monooxygenase (styA and styB), styrene oxide isomerase (st...

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Veröffentlicht in:Journal of microbiology and biotechnology 2006-07, Vol.16 (7), p.1032-1040
Hauptverfasser: Park, M.S. (Pusan National University, Busan, Republic of Korea), Bae, J.W. (Pusan National University, Busan, Republic of Korea), Han, J.H. (Pusan National University, Busan, Republic of Korea), Lee, E.Y. (Pusan National University, Busan, Republic of Korea), Lee, S.G. (Pusan National University, Busan, Republic of Korea), Park, S.H. (Pusan National University, Busan, Republic of Korea), E-mail: parksh@pusan.ac.kr
Format: Artikel
Sprache:eng
Schlagworte:
(S)-styrene oxide
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
induction condition
PSEUDOMONAS PUTIDA
styrene monooxygenase
whole-cell biocatalyst
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Zusammenfassung:Some Pseudomonas species can grow on styrene as a sole carbon and energy source. From the new isolate Pseudomonas putida SN1, the genes for styrene catabolism were cloned and sequenced. They were composed of four structural genes for styrene monooxygenase (styA and styB), styrene oxide isomerase (styC), and phenylacetaldehyde dehydrogenase (styD), along with two genes for the regulatory system (styS and styR). All the genes showed high DNA sequence (91% to 99%) and amino acid sequence (94% to 100%) similarities with the corresponding genes of the previously reported styrene-degrading Pseudomonas strains.
ISSN:1017-7825