Inulooligosaccharide Production from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Endoinulinase
The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The const...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2006-03, Vol.16 (3), p.360-367 |
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| container_title | Journal of microbiology and biotechnology |
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| creator | Kim, H.C. (Dongeui University, Busan, Republic of Korea) Kim, H.J. (Dongeui University, Busan, Republic of Korea) Choi, W.B. (Dongeui University, Busan, Republic of Korea) Nam, S.W. (Dongeui University, Busan, Republic of Korea), E-mail: swnam@deu.ac.kr |
| description | The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTENIU (8.5kb), was then introduced to S. cerevisiae EBY100 cells and the yeast transformants selected on synthetic defined media lacking uracil and inulin-containing media. |
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(Dongeui University, Busan, Republic of Korea) ; Kim, H.J. (Dongeui University, Busan, Republic of Korea) ; Choi, W.B. (Dongeui University, Busan, Republic of Korea) ; Nam, S.W. (Dongeui University, Busan, Republic of Korea), E-mail: swnam@deu.ac.kr</creator><creatorcontrib>Kim, H.C. (Dongeui University, Busan, Republic of Korea) ; Kim, H.J. (Dongeui University, Busan, Republic of Korea) ; Choi, W.B. (Dongeui University, Busan, Republic of Korea) ; Nam, S.W. (Dongeui University, Busan, Republic of Korea), E-mail: swnam@deu.ac.kr</creatorcontrib><description>The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTENIU (8.5kb), was then introduced to S. cerevisiae EBY100 cells and the yeast transformants selected on synthetic defined media lacking uracil and inulin-containing media.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; cell-surface display ; Endoinulinase ; Fundamental and applied biological sciences. 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(Dongeui University, Busan, Republic of Korea), E-mail: swnam@deu.ac.kr</creatorcontrib><title>Inulooligosaccharide Production from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Endoinulinase</title><title>Journal of microbiology and biotechnology</title><description>The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTENIU (8.5kb), was then introduced to S. cerevisiae EBY100 cells and the yeast transformants selected on synthetic defined media lacking uracil and inulin-containing media.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell-surface display</subject><subject>Endoinulinase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>INULIN</subject><subject>INULINA</subject><subject>INULINE</subject><subject>inulooligosaccharide</subject><subject>SACCHAROMYCES CEREVISIAE</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNotzE9LwzAYgPEeFJzTjyDk4rGQNP_ao8ypw4Fi9Tzevn0zI1kzkk7ot3c6T8_lx3NWzAQXtrR1pS-Ky5y_ODeiqs2sGFfDIcQY_DZmQPyE5Htiryn2Bxx9HJhLccd-kR9YN7H2hOJuQsoMKdG3zx6ItWOCI7n3eR9g8sOWLSiEsj0kB0hsOfTR_10g01Vx7iBkuv7vvPh4WL4vnsr1y-NqcbcuXcXtWDa90I1SujGuE9oqpE4K5QQaYzojQRHXQjtJgIBSNl0D1GhVd2CRK1vLeXF7-u4hIwSXYECfN_vkd5CmjbBG6NrYo7s5OQdxA9t0NM9vFeeW88oIKX8AHZRiMg</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Kim, H.C. (Dongeui University, Busan, Republic of Korea)</creator><creator>Kim, H.J. (Dongeui University, Busan, Republic of Korea)</creator><creator>Choi, W.B. (Dongeui University, Busan, Republic of Korea)</creator><creator>Nam, S.W. 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| ispartof | Journal of microbiology and biotechnology, 2006-03, Vol.16 (3), p.360-367 |
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| language | eng |
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| subjects | Biological and medical sciences Biotechnology cell-surface display Endoinulinase Fundamental and applied biological sciences. Psychology INULIN INULINA INULINE inulooligosaccharide SACCHAROMYCES CEREVISIAE |
| title | Inulooligosaccharide Production from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Endoinulinase |
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