Inulooligosaccharide Production from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Endoinulinase

Inulooligosaccharide Production from Inulin by Saccharomyces cerevisiae Strain Displaying Cell-Surface Endoinulinase

The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The const...

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Veröffentlicht in:Journal of microbiology and biotechnology 2006-03, Vol.16 (3), p.360-367
Hauptverfasser: Kim, H.C. (Dongeui University, Busan, Republic of Korea), Kim, H.J. (Dongeui University, Busan, Republic of Korea), Choi, W.B. (Dongeui University, Busan, Republic of Korea), Nam, S.W. (Dongeui University, Busan, Republic of Korea), E-mail: swnam@deu.ac.kr
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
cell-surface display
Endoinulinase
Fundamental and applied biological sciences. Psychology
INULIN
INULINA
INULINE
inulooligosaccharide
SACCHAROMYCES CEREVISIAE
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Zusammenfassung:The endoinulinase gene (inu1) from Pseudomonas mucidolens was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The inu1 gene of P. mucidolens was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTENIU (8.5kb), was then introduced to S. cerevisiae EBY100 cells and the yeast transformants selected on synthetic defined media lacking uracil and inulin-containing media.
ISSN:1017-7825