Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease

We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3/cnl-1-like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays off...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Clinical Microbiology 2006-03, Vol.44 (3), p.1127-1131
Hauptverfasser:	Bennett, Désirée E, Cafferkey, Mary T
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Base Sequence Biological and medical sciences Carrier State - microbiology DNA Primers - genetics DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Meningococcal Infections - microbiology Microbiology Miscellaneous Neisseria meningitidis Neisseria meningitidis - classification Neisseria meningitidis - genetics Neisseria meningitidis - pathogenicity Neisseria meningitidis, Serogroup A - classification Neisseria meningitidis, Serogroup A - genetics Neisseria meningitidis, Serogroup A - pathogenicity Neisseria meningitidis, Serogroup B - classification Neisseria meningitidis, Serogroup B - genetics Neisseria meningitidis, Serogroup B - pathogenicity Neisseria meningitidis, Serogroup C - classification Neisseria meningitidis, Serogroup C - genetics Neisseria meningitidis, Serogroup C - pathogenicity Neisseria meningitidis, Serogroup W-135 - classification Neisseria meningitidis, Serogroup W-135 - genetics Neisseria meningitidis, Serogroup W-135 - pathogenicity Neisseria meningitidis, Serogroup Y - classification Neisseria meningitidis, Serogroup Y - genetics Neisseria meningitidis, Serogroup Y - pathogenicity Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Sensitivity and Specificity Serotyping - methods Serotyping - statistics & numerical data Virulence - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3/cnl-1-like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays offer a rapid and simple method of serogrouping N. meningitidis.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.44.3.1127-1131.2006