Cloning and Characterization of the pyrH Gene Encoding UMP-Kinase from Lactobacillus reuteri ATCC 55739

From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase compose...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2005-06, Vol.15 (3), p.525-531
Hauptverfasser:	Park, J.Y. (Gyeongsang National University, Jinju, Republic of Korea), Nam, S.J. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), Jeong, S.J. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.K. (Gyeongsang National University, Jinju, Republic of Korea), Ha, Y.L. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: jeonghkm@nongae.gsnu.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology LACTOBACILLUS Lactobacillus reuteri operon pyrH ribosomal recycling factor UMP-kinase
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Zusammenfassung:	From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively.
ISSN:	1017-7825