Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococcus aureus Strains

The mecA gene was lost in 36 (14.4%) of 250 methicillin-resistant Staphylococcus aureus isolates after 2 years of storage at -80°C with the Microbank system (Pro-lab Diagnostics, Austin, Tex.). Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has...

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Veröffentlicht in:	Journal of Clinical Microbiology 2005-03, Vol.43 (3), p.1361-1365
Hauptverfasser:	van Griethuysen, Arjanne, van Loo, Inge, van Belkum, Alex, Vandenbroucke-Grauls, Christina, Wannet, Wim, van Keulen, Peter, Kluytmans, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacteriology Biological and medical sciences Blotting, Southern Culture Media Freezing Fundamental and applied biological sciences. Psychology Infectious diseases Medical sciences Methicillin Resistance Microbiology Miscellaneous Penicillin-Binding Proteins Polymerase Chain Reaction Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics
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Beschreibung
Zusammenfassung:	The mecA gene was lost in 36 (14.4%) of 250 methicillin-resistant Staphylococcus aureus isolates after 2 years of storage at -80°C with the Microbank system (Pro-lab Diagnostics, Austin, Tex.). Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.43.3.1361-1365.2005