Structural analysis of water-soluble and -insoluble β-glucans of whole-grain oats and barley
Water-soluble and water-insoluble (1→3),(1→4)-β- d-glucans were isolated from whole-grain oats and barley and digested with lichenase. The oligosaccharides thus produced were analysed using anion-exchange chromatography with pulse-amperometric detection (HPAEC-PAD). The FT-IR, 1H NMR and solid-state...
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Veröffentlicht in: | Carbohydrate polymers 2004-11, Vol.58 (3), p.267-274 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Water-soluble and water-insoluble (1→3),(1→4)-β-
d-glucans were isolated from whole-grain oats and barley and digested with lichenase. The oligosaccharides thus produced were analysed using anion-exchange chromatography with pulse-amperometric detection (HPAEC-PAD). The FT-IR,
1H NMR and solid-state
13C CP-MAS NMR spectra of the β-glucans were also measured. Analyses of the ratio of oligosaccharides with degrees of polymerisation 3 and 4 (DP3:DP4) showed small structural differences between oats and barley and between the water-soluble and water-insoluble β-glucans. The molar masses analysed using the SEC-HPLC method were 500,000
g/mol for the soluble β-glucans of both oats and barley and |
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ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2004.06.041 |