Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine
The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but...
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Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2459-2462 |
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container_title | Bioscience, biotechnology, and biochemistry |
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creator | MATSUOKA, Hideaki OISHI, Kanenari WATANABE, Masaaki KOZONE, Ikuko SAITO, Mikako IGIMI, Shizunobu |
description | The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake. |
doi_str_mv | 10.1271/bbb.67.2459 |
format | Article |
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Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.67.2459</identifier><identifier>PMID: 14646209</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject><![CDATA[4-Chloro-7-nitrobenzofurazan - analogs & derivatives ; Aeromonas hydrophila - growth & development ; Bacteria - growth & development ; Biological and medical sciences ; Deoxyglucose - analogs & derivatives ; Escherichia coli - growth & development ; Fluorescent Dyes ; fluorescent glucose ; fluorescent glycine ; foodborne pathogenic bacteria ; Fundamental and applied biological sciences. Psychology ; Glycine - analogs & derivatives ; Listeria monocytogenes - growth & development ; Pseudomonas aeruginosa - growth & development ; Salmonella typhimurium - growth & development ; viable cell detection]]></subject><ispartof>Bioscience, biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2459-2462</ispartof><rights>2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2003</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c619t-d971648ef141d2caa3fff0994f98ed7ad36ad2bcfc8e4d60763eb7b4f3dbb0fb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15405214$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14646209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MATSUOKA, Hideaki</creatorcontrib><creatorcontrib>OISHI, Kanenari</creatorcontrib><creatorcontrib>WATANABE, Masaaki</creatorcontrib><creatorcontrib>KOZONE, Ikuko</creatorcontrib><creatorcontrib>SAITO, Mikako</creatorcontrib><creatorcontrib>IGIMI, Shizunobu</creatorcontrib><title>Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.</description><subject>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</subject><subject>Aeromonas hydrophila - growth & development</subject><subject>Bacteria - growth & development</subject><subject>Biological and medical sciences</subject><subject>Deoxyglucose - analogs & derivatives</subject><subject>Escherichia coli - growth & development</subject><subject>Fluorescent Dyes</subject><subject>fluorescent glucose</subject><subject>fluorescent glycine</subject><subject>foodborne pathogenic bacteria</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycine - analogs & derivatives</subject><subject>Listeria monocytogenes - growth & development</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Salmonella typhimurium - growth & development</subject><subject>viable cell detection</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtLxDAQBvAgiq6Pk3cpiF6ka9KmSXOU9QmCFxVvIY8JVtJGkxbZ_97IrgjiaeDjN8PwIXRI8JxUnJxrreeMzyvaiA00IzXlJROUb6IZFoSVLW3IDtpN6Q3jHDRkG-0QyiirsJihl-dOaQ_FArwvLmEEM3ZhKPSyGF9zGnrdDWCLpwRFcMW1n0KEZGAYixs_mZBjNdg_-dLknX205ZRPcLCee-jp-upxcVveP9zcLS7uS8OIGEsrOGG0BUcosZVRqnbOYSGoEy1YrmzNlK20caYFahnmrAbNNXW11Ro7Xe-h09Xd9xg-Jkij7Lv8iPdqgDAlyfPhtqp5hsd_4FuY4pB_k4RSQRssWpzV2UqZGFKK4OR77HoVl5Jg-V23zHVLxuV33VkfrW9Ougf7a9f9ZnCyBioZ5V1Ug-nSr2sobipCs2Mr1w0uxF59huitHNXSh_izVP_3wRfcOpqd</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>MATSUOKA, Hideaki</creator><creator>OISHI, Kanenari</creator><creator>WATANABE, Masaaki</creator><creator>KOZONE, Ikuko</creator><creator>SAITO, Mikako</creator><creator>IGIMI, Shizunobu</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine</title><author>MATSUOKA, Hideaki ; OISHI, Kanenari ; WATANABE, Masaaki ; KOZONE, Ikuko ; SAITO, Mikako ; IGIMI, Shizunobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c619t-d971648ef141d2caa3fff0994f98ed7ad36ad2bcfc8e4d60763eb7b4f3dbb0fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</topic><topic>Aeromonas hydrophila - growth & development</topic><topic>Bacteria - growth & development</topic><topic>Biological and medical sciences</topic><topic>Deoxyglucose - analogs & derivatives</topic><topic>Escherichia coli - growth & development</topic><topic>Fluorescent Dyes</topic><topic>fluorescent glucose</topic><topic>fluorescent glycine</topic><topic>foodborne pathogenic bacteria</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycine - analogs & derivatives</topic><topic>Listeria monocytogenes - growth & development</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Salmonella typhimurium - growth & development</topic><topic>viable cell detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUOKA, Hideaki</creatorcontrib><creatorcontrib>OISHI, Kanenari</creatorcontrib><creatorcontrib>WATANABE, Masaaki</creatorcontrib><creatorcontrib>KOZONE, Ikuko</creatorcontrib><creatorcontrib>SAITO, Mikako</creatorcontrib><creatorcontrib>IGIMI, Shizunobu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUOKA, Hideaki</au><au>OISHI, Kanenari</au><au>WATANABE, Masaaki</au><au>KOZONE, Ikuko</au><au>SAITO, Mikako</au><au>IGIMI, Shizunobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>67</volume><issue>11</issue><spage>2459</spage><epage>2462</epage><pages>2459-2462</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. 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subjects | 4-Chloro-7-nitrobenzofurazan - analogs & derivatives Aeromonas hydrophila - growth & development Bacteria - growth & development Biological and medical sciences Deoxyglucose - analogs & derivatives Escherichia coli - growth & development Fluorescent Dyes fluorescent glucose fluorescent glycine foodborne pathogenic bacteria Fundamental and applied biological sciences. Psychology Glycine - analogs & derivatives Listeria monocytogenes - growth & development Pseudomonas aeruginosa - growth & development Salmonella typhimurium - growth & development viable cell detection |
title | Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine |
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