Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine

The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2459-2462
Hauptverfasser:	MATSUOKA, Hideaki, OISHI, Kanenari, WATANABE, Masaaki, KOZONE, Ikuko, SAITO, Mikako, IGIMI, Shizunobu
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Chloro-7-nitrobenzofurazan - analogs & derivatives Aeromonas hydrophila - growth & development Bacteria - growth & development Biological and medical sciences Deoxyglucose - analogs & derivatives Escherichia coli - growth & development Fluorescent Dyes fluorescent glucose fluorescent glycine foodborne pathogenic bacteria Fundamental and applied biological sciences. Psychology Glycine - analogs & derivatives Listeria monocytogenes - growth & development Pseudomonas aeruginosa - growth & development Salmonella typhimurium - growth & development viable cell detection
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Zusammenfassung:	The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.67.2459