Characterization of Humanized Antibody produced by Apoptosis-Resistant CHO Cells under Sodium Butyrate-Induced Condition

Overexpression of human Bcl-2 protein in recombinant Chinese hamster ovary cells producing humanized antibody considerably suppressed sodium butyrate(NaBu)-induced apoptosis during batch culture buy using commercially available serum-free medium, which extended the culture longevity. Due to the exte...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2003-12, Vol.13 (6), p.926-936
Hauptverfasser:	Kim, N.S, Chang, K.H, Chung, B.S, Kim, S.H, Kim, J.H, Lee, G.M. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea)
Format:	Artikel
Sprache:	eng
Schlagworte:	apoptosis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology sodium
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Zusammenfassung:	Overexpression of human Bcl-2 protein in recombinant Chinese hamster ovary cells producing humanized antibody considerably suppressed sodium butyrate(NaBu)-induced apoptosis during batch culture buy using commercially available serum-free medium, which extended the culture longevity. Due to the extended culture longevity provided by the anti-apoptotic effect of Bcl-2 overexpression, was 2 times higher than that of the SH2-0.32-bcl-2 culture in the presence of NaBu.
ISSN:	1017-7825