Selectivity Studies of the Benzene cis -Dihydrodiol Dehydrogenase Enzyme from Pseudomonas putida ML2 with Vicinal Diol Substrates

The enantiopure (1 S, 2 S )- cis -dihydrodiol metabolites 2B - 5B have been obtained in low yield from the corresponding monosubstituted halobenzene substrates 2A - 5A, using a wild-type strain of Pseudomonas putida (ML2) containing benzene dioxygenase (BDO). Benzene cis -dihydrodiol dehydrogenase (...

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Veröffentlicht in:	Biocatalysis and biotransformation 2002, Vol.20 (4), p.257-264
Hauptverfasser:	Allen, Christopher C.R., Walker, Claire E., Sharma, Narain D., Kerley, Nuala A., Boyd, Derek R., Dalton, Howard
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzene cis -dihydrodiol Dehydrogenase Biocatalysis Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Biotransformations Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Pseudomonas putida Pseudomonas Putida Ml2
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Zusammenfassung:	The enantiopure (1 S, 2 S )- cis -dihydrodiol metabolites 2B - 5B have been obtained in low yield from the corresponding monosubstituted halobenzene substrates 2A - 5A, using a wild-type strain of Pseudomonas putida (ML2) containing benzene dioxygenase (BDO). Benzene cis -dihydrodiol dehydrogenase (BCD) from P. putida ML2 and naphthalene cis -dihydrodiol dehydrogenase (NCD) from P. putida 8859 were purified and used in a comparative study of the stereoselective biotransformation of cis -dihydrodiol enantiomers 2B - 5B. The BCD and NCD enzymes were found to accept cis -dihydrodiol enantiomers of monosubstituted benzene cis -dihydrodiol substrates 2B - 5B of opposite absolute configuration. The acyclic alkene 1,2-diols 10 - 17 were also found to be acceptable substrates for BCD.
ISSN:	1024-2422 1029-2446
DOI:	10.1080/10242420290029454