Establishment of callus and cell suspension cultures of Opuntia ficus-indica

Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 micromolar 6-furfurylaminopu...

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Veröffentlicht in:	Plant cell, tissue and organ culture tissue and organ culture, 1999, Vol.58 (2), p.155-157
Hauptverfasser:	Llamoca-Zarate, R.M, Studart-Guimaraes, C, Landsmann, J, Campos, F.A.P
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-D aromatic acids Biological and medical sciences Biotechnology callus casein hydrolysate cell suspension culture cotyledons culture media Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures explants Fundamental and applied biological sciences. Psychology hypocotyls Methods. Procedures. Technologies micropropagation Opuntia ficus-indica Plant cells and fungal cells purines sucrose tissue culture
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Zusammenfassung:	Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 micromolar 6-furfurylaminopurine, 2.3 micromolar 2,4-dichlorophenoxyacetic acid, 1.0 micromolar 4-amino 3,5,6-trichloropicolinic acid, 400 mg/l casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.
ISSN:	0167-6857 1573-5044
DOI:	10.1023/A:1006315729266