Interphase FISH for rapid identification of a Down syndrome animal model

Mouse models of Down syndrome (trisomy 21, DS) have been developed to study the consequences of triplication of regions syntenic to distal human chromosome 21. We report a technique for the identification of segmental trisomy 16 (Ts65Dn) and diploid progeny at any age using interphase fluorescence i...

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Veröffentlicht in:	Cytogenetic and genome research 1999-01, Vol.86 (3-4), p.285-287
Hauptverfasser:	Strovel, J., Stamberg, J., Yarowsky, P.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Cytogenetics and Comparative Mapping Animals Biological and medical sciences chromosome 21 Crosses, Genetic Cytogenetics Disease Models, Animal Down Syndrome - genetics Down's syndrome Female Fibroblasts - cytology Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Humans In Situ Hybridization, Fluorescence Interphase Male Mice Mice, Inbred C3H Mice, Inbred C57BL Vertebrata
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Beschreibung
Zusammenfassung:	Mouse models of Down syndrome (trisomy 21, DS) have been developed to study the consequences of triplication of regions syntenic to distal human chromosome 21. We report a technique for the identification of segmental trisomy 16 (Ts65Dn) and diploid progeny at any age using interphase fluorescence in situ hybridization (FISH) of uncultured tail fibroblasts. Our technique is faster and less expensive than blood karyotyping.
ISSN:	1424-8581 0301-0171 1424-859X
DOI:	10.1159/000015320