Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi

Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi

The oleaginous yeast, Lipomyces starkeyi, is an excellent lipid producer with great industrial potential. However, methods for molecular breeding have not been established for L. starkeyi. We describe the development of a system for targeted rDNA integration of multiple copies of a gene into L. star...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2015-03, Vol.79 (3), p.512-515
Hauptverfasser: Oguro, Yoshifumi, Yamazaki, Harutake, Shida, Yosuke, Ogasawara, Wataru, Takagi, Masamichi, Takaku, Hiroaki
Format: Artikel
Sprache:eng
Schlagworte:
DNA, Ribosomal - genetics
Gene Expression
Genetic Engineering - methods
Genome, Fungal - genetics
Lipomyces - genetics
Lipomyces starkeyi
multicopy integration
oleaginous yeast
Polyethylene Glycols - chemistry
transformation
Transformation, Genetic
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Zusammenfassung:The oleaginous yeast, Lipomyces starkeyi, is an excellent lipid producer with great industrial potential. However, methods for molecular breeding have not been established for L. starkeyi. We describe the development of a system for targeted rDNA integration of multiple copies of a gene into L. starkeyi genome by spheroplast-polyethylene glycol transformation.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2014.982504