Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allo...

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Veröffentlicht in:	Journal of microbiological methods 2005, Vol.60 (1), p.41-46
Hauptverfasser:	Clingenpeel, Scott R., Keener, William K., Keller, Caron R., De Jesus, Karl, Howard, M. Hope, Watwood, Mary E.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Ethynylbenzoate AROMATICS Bacteriology Benzoates - metabolism Biological and medical sciences Bioremediation Chromogenic ENVIRONMENTAL SCIENCES, 60 - APPLIED LIFE SCIENCES ENZYMES FISSION Fluorescent Dyes - metabolism Fluorogenic Fundamental and applied biological sciences. Psychology Microbiology Microscopy, Fluorescence Miscellaneous Oxygenases - metabolism PROBES PSEUDOMONAS Pseudomonas putida - enzymology Pseudomonas putida - metabolism PYROCATECHOL TOLUENE Toluene - metabolism
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Beschreibung
Zusammenfassung:	3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2004.08.018