Structure of Pumilio Reveals Similarity between RNA and Peptide Binding Motifs
Translation regulation plays an essential role in the differentiation and development of animal cells. One well-studied case is the control of hunchback mRNA during early Drosophila embryogenesis by the trans-acting factors Pumilio, Nanos, and Brain Tumor. We report here a crystal structure of the c...
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Veröffentlicht in: | Cell 2001-04, Vol.105 (2), p.281-289 |
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Sprache: | eng |
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Zusammenfassung: | Translation regulation plays an essential role in the differentiation and development of animal cells. One well-studied case is the control of
hunchback mRNA during early
Drosophila embryogenesis by the trans-acting factors Pumilio, Nanos, and Brain Tumor. We report here a crystal structure of the critical region of Pumilio, the Puf domain, that organizes a multivalent repression complex on the 3′ untranslated region of
hunchback mRNA. The structure reveals an extended, rainbow shaped molecule, with tandem helical repeats that bear unexpected resemblance to the armadillo repeats in β-catenin and the HEAT repeats in protein phosphatase 2A. Based on the structure and genetic experiments, we identify putative interaction surfaces for
hunchback mRNA and the cofactors Nanos and Brain Tumor. This analysis suggests that similar features in helical repeat proteins are used to bind extended peptides and RNA. |
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ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/S0092-8674(01)00318-X |