Electrospray Mass Spectrometric Study of Protein-Ketone Equilibria in Solution

The electrospray mass spectra of basic protein solutions containing ketones exhibit ions that are attributable to protein-ketone adducts whose relative abundances are in accordance with the relative concentrations of these adducts in solution. Excellent agreement with known numbers was observed for...

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Veröffentlicht in:	Analytical chemistry (Washington) 1994-10, Vol.66 (20), p.3289-3296
Hauptverfasser:	Le Blanc, J. C. Y, Siu, K. W. M, Guevremont, Roger
Format:	Artikel
Sprache:	eng
Schlagworte:	400102 - Chemical & Spectral Procedures 400201 - Chemical & Physicochemical Properties ADDUCTS ALKYLATION CHEMICAL ANALYSIS CHEMICAL REACTIONS Chemistry DISPERSIONS EQUILIBRIUM IMINES INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY Ions KETONES MASS SPECTROSCOPY MATHEMATICS MIXTURES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PROTEINS SCHIFF BASES Scientific imaging SOLUTIONS SPECTROSCOPY STATISTICS
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Beschreibung
Zusammenfassung:	The electrospray mass spectra of basic protein solutions containing ketones exhibit ions that are attributable to protein-ketone adducts whose relative abundances are in accordance with the relative concentrations of these adducts in solution. Excellent agreement with known numbers was observed for all 18 proteins measured.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00092a003