Big blue transgenic mouse lacl mutation analysis

In this report the authors describe a rapid general method for mutant blue plaque molecular analysis. The mutant analysis discussed here resulted from radon inhalation exposure. The described method circumvents Stratagene's plasmid isolation and ensuing sequence analysis of the entire lac1 gene. The authors have adapted the polymerase chain reaction (PCR) method and single-strand conformation polymorphism (SSCP) analysis for localizing mutations within the lac1 coding region. Three overlapping PCR products of approximately 450 bp representing the entire lac1 coding region are used for SSCP analysis. Those PCR products with an altered SSCP electrophoretic migration focus the mutation to a smaller region of the lac1 gene that is analyzed by direct cycle sequencing. 5 refs., 1 fig.