Native fluorescence detection of nucleic acids and DNA restriction fragments in capillary electrophoresis

A sensitive laser-induced fluorescence (LIF) detection scheme for native nucleic acids and DNA restriction fragments separated by capillary elecrophoresis (CE) is described. This system enables the sensitive detection of nucleic-acid-containing compounds without the need for fluroescence labeling.

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Bibliographische Detailangaben
Veröffentlicht in:	Analytical chemistry (Washington) 1993-01, Vol.65 (2), p.153-157
Hauptverfasser:	Milofsky, Robert E, Yeung, Edward S
Format:	Artikel
Sprache:	eng
Schlagworte:	400102 - Chemical & Spectral Procedures 550200 - Biochemistry Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry BASIC BIOLOGICAL SCIENCES Biochemistry Biological and medical sciences BLOOD VESSELS BODY CAPILLARIES CARDIOVASCULAR SYSTEM Deoxyribonucleic acid DNA DNA - analysis DNA Restriction Enzymes ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel EMISSION SPECTROSCOPY Fluorescence FLUORESCENCE SPECTROSCOPY Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY Ions LASER SPECTROSCOPY Lasers NUCLEIC ACIDS Oligodeoxyribonucleotides - analysis ORGANIC COMPOUNDS ORGANS QUENCHING Scientific imaging SENSITIVITY SPECTROSCOPY
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Beschreibung
Zusammenfassung:	A sensitive laser-induced fluorescence (LIF) detection scheme for native nucleic acids and DNA restriction fragments separated by capillary elecrophoresis (CE) is described. This system enables the sensitive detection of nucleic-acid-containing compounds without the need for fluroescence labeling.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00050a011