Effects of acetylene on hydrogenases from the sulfate reducing and methanogenic bacteria

The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase b...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-05, Vol.161 (1), p.127-133
Hauptverfasser:	He, S.-H., Woo, S.B., DerVartanian, D.V., Le Gall, J., Peck, H.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	560300 - Chemicals Metabolism & Toxicology ACETYLENE Acetylene - pharmacology ALKYNES Analytical, structural and metabolic biochemistry BACTERIA Biological and medical sciences BIOLOGICAL EFFECTS Biotechnology Desulfovibrio Desulfovibrio - drug effects Desulfovibrio - enzymology ELECTRON SPIN RESONANCE Electron Spin Resonance Spectroscopy ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors Euryarchaeota - drug effects Euryarchaeota - enzymology Fundamental and applied biological sciences. Psychology HYDROCARBONS hydrogenase Hydrogenase - antagonists & inhibitors Hydrogenase - metabolism HYDROGENASES INHIBITION MAGNETIC RESONANCE Methanococcus voltae METHANOGENIC BACTERIA Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology MICROORGANISMS ORGANIC COMPOUNDS OXIDOREDUCTASES PROTEINS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RESONANCE SULFATE-REDUCING BACTERIA
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Zusammenfassung:	The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase being 10–50 fold more sensitive than the (NiFeSe) hydrogenase. In addition the Ni(III) EPR signal (g∼2.3) of the “as isolated” (NiFe) hydrogenase was significantly decreased in intensity upon exposure to acetylene.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(89)91570-2