Evaluation of dibromonitrosobenzene sulfonate as a spin trap in biological systems

In the present study dibromonitrosobenzene sulfonate (DBNBS) was examined for its suitability for spin trapping for ESR detection of superoxide radicals in biological systems. This nitroso spin trap recently has been reported to yield very persistent spin adducts with O 2 −· as well as with various...

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Veröffentlicht in:Free radical biology & medicine 1989, Vol.7 (1), p.37-43
Hauptverfasser: Samuni, Ayelet, Samuni, Amram, Swartz, Harold M.
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Sprache:eng
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Zusammenfassung:In the present study dibromonitrosobenzene sulfonate (DBNBS) was examined for its suitability for spin trapping for ESR detection of superoxide radicals in biological systems. This nitroso spin trap recently has been reported to yield very persistent spin adducts with O 2 −· as well as with various carbon-centered radicals. In the present work the possible toxicity of DBNBS, the partitioning of its spin adducts into cells, and the stability of the adducts and the parent compound inside cells were studied. no significant toxicity was found. In cellular systems, however, DBNBS did not produce detectable adducts with O 2 −·; it also did not detectably trap superoxide generated in the xanthine/xanthine oxidase system. Both DBNBS and a DBNBS adduct preformed extracellularly and then added to cell suspensions were rapidly metabolized by cells. Intracellular spin adducts were not detected under any condition. Evidently, in spiute of its promising features, DBNBS will not be useful for spin trapping radicals in cellular systems or for detecting superoxide radicals in any biological system.
ISSN:0891-5849
1873-4596
DOI:10.1016/0891-5849(89)90098-1