DNA sequences and expression in Streptomyces lividans of an exogluycanase gene and an endoglucanase gene from Thermomonospora fusca

The authors have been studying the mechanism of cellulose degradation by the cellulaces of the actinomycete Thermomonospora fusca, a thermophylic, filamentous soil bacterium. Six structurally and functionally distinct cellulases, E1 to E6 have been identified and purified. In the present study the E...

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Veröffentlicht in:	Applied and environmental microbiology 1993-09, Vol.59:9
Hauptverfasser:	Jung, E.D., Lao, G., Irwin, D., Barr, B.K., Benjamin, A., Wilson, D.B.
Format:	Artikel
Sprache:	eng
Schlagworte:	560300 > Chemicals Metabolism & Toxicology BACTERIA BASIC BIOLOGICAL SCIENCES BIODEGRADATION BIOLOGICAL PATHWAYS CARBOHYDRATES CELLULASE CELLULOSE CHEMICAL REACTIONS DECOMPOSITION DNA DNA SEQUENCING ECOLOGICAL CONCENTRATION ENZYMES ESCHERICHIA COLI GENES GLYCOSYL HYDROLASES HYDROLASES MICROORGANISMS NUCLEIC ACIDS O-GLYCOSYL HYDROLASES ORGANIC COMPOUNDS POLYSACCHARIDES PROTEINS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT SACCHARIDES SOILS STREPTOMYCES STRUCTURAL CHEMICAL ANALYSIS 550400 > Genetics
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Zusammenfassung:	The authors have been studying the mechanism of cellulose degradation by the cellulaces of the actinomycete Thermomonospora fusca, a thermophylic, filamentous soil bacterium. Six structurally and functionally distinct cellulases, E1 to E6 have been identified and purified. In the present study the E1 and E4 genes have been sequenced and introduced into both Streptomyces lividans and Escherichia coli. The products of digestion of cellutriose, cellotetraose, cellopentaose, and swollen cellulose revealed quite different product profiles and specificities for E1 and E4. 41 refs., 8 figs., 4 tabs.
ISSN:	0099-2240 1098-5336