Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs

Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synth...

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Veröffentlicht in:Biochemical and biophysical research communications 2018-01, Vol.495 (1), p.401-407
Hauptverfasser: Nakajima, Kazuki, Kizuka, Yasuhiko, Yamaguchi, Yoshiki, Hirabayashi, Yoshio, Takahashi, Kazuo, Yuzawa, Yukio, Taniguchi, Naoyuki
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Sprache:eng
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Zusammenfassung:Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions. •UDP-Man was detected in mammals for the first time using LC-MS analysis.•UDP-Man was found in cell lines by monitoring characteristic fragment ions.•Cellular UDP-Man levels are strongly elevated in a mannose dose-dependent manner.•Hypothalamus and neocortex contain more UDP-Man than other regions and organs.•UDP-Man likely regulates mannosylation in specific organs or conditions.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2017.10.173