Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells

We studied the influence of magnetite nanoparticles (FeO•Fe 2 O 3 ) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes ( BA , B2M , PPIA , UBC , and YWHAZ ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2018-12, Vol.166 (2), p.264-267
Hauptverfasser:	Fomina, S. G., Novikov, D. V., Krasnogorova, N. V., Novikov, V. V., Pleskova, S. N., Karaulov, A. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	14-3-3 Proteins - genetics 14-3-3 Proteins - metabolism 3-Mercaptopropionic Acid - chemistry 60 APPLIED LIFE SCIENCES Actins - genetics Actins - metabolism beta 2-Microglobulin - genetics beta 2-Microglobulin - metabolism Biomedical and Life Sciences Biomedicine Cadmium Compounds - pharmacology CADMIUM SELENIDES Cell Biology Ferric Compounds - pharmacology Gene Expression Regulation - drug effects GENES Humans Internal Medicine IRON OXIDES Laboratory Medicine Leukocytes (neutrophilic) Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism LYMPHOCYTES MAGNETITE Magnetite Nanoparticles - chemistry MESSENGER-RNA MONOCYTES Nanoparticles NEUTROPHILS Pathology Peptidylprolyl Isomerase - genetics Peptidylprolyl Isomerase - metabolism Peripheral blood POLYMERASE CHAIN REACTION Polymerase Chain Reaction - standards Primary Cell Culture QUANTUM DOTS Quantum Dots - chemistry Reference Standards Reverse transcription Selenium Compounds - pharmacology Sulfides - pharmacology Ubiquitin C - genetics Ubiquitin C - metabolism Western blotting Zinc Compounds - pharmacology ZINC SULFIDES
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Zusammenfassung:	We studied the influence of magnetite nanoparticles (FeO•Fe 2 O 3 ) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes ( BA , B2M , PPIA , UBC , and YWHAZ ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-018-4329-x