New fructan — the O-specific polysaccharide from Escherichia coli O92

The structure of the O-specific polysaccharide (O-antigen) from Escherichia coli O92 was established. The acid-labile polysaccharide was produced by O-deacylation of the lipopolysaccharide under mild alkaline conditions. The methylation analysis combined with 1D and 2D 1 H and 13 C NMR spectroscopy...

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Veröffentlicht in:Russian chemical bulletin 2017-07, Vol.66 (7), p.1304-1307
Hauptverfasser: Senchenkova, S. N., Xu, Guangnan, Qi, Yanfen, Perepelov, A. V., Shashkov, A. S., Liu, Bin, Knirel, Yu. A.
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Sprache:eng
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Zusammenfassung:The structure of the O-specific polysaccharide (O-antigen) from Escherichia coli O92 was established. The acid-labile polysaccharide was produced by O-deacylation of the lipopolysaccharide under mild alkaline conditions. The methylation analysis combined with 1D and 2D 1 H and 13 C NMR spectroscopy demonstrated that the O-specific polysaccharide chain of the lipopolysaccharide is a linear fructan composed of mono-О-acetylated disaccharide repeating units having the structure →1)-β- d -Fru f 3Ac-(2→3)-β- d -Fru f -(2→. To the best of our knowledge, this fructan has been hitherto unknown in bacteria.
ISSN:1066-5285
1573-9171
DOI:10.1007/s11172-017-1889-2