Wnt/β-catenin signaling activates nephronectin expression in osteoblasts

Nephronectin (Npnt), an extracellular matrix protein, is considered to play critical roles as an adhesion molecule in the development and functions of various organs and tissues, such as the kidneys and bone. In the present study, we found that Wnt3a strongly enhanced Npnt mRNA expression in osteoblast-like MC3T3-E1 cells, while it also induced an increase in Npnt gene expression in both time- and dose-dependent manners via the Wnt/β-catenin signaling pathway. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression. •Wnt3a enhances nephronectin gene expression.•Nephronectin gene induction by Wnt3a is occurred by time- and dose-dependent manner.•Expression of nephronectin is regulated via β-catenin activation.