miR-409-3p suppresses breast cancer cell growth and invasion by targeting Akt1

Altered levels and functions of microRNAs (miRNAs) are correlated with carcinogenesis. While miR-409-3p has been shown to play important roles in several cancer types, its function in the context of breast cancer (BC) remains unknown. In this study, miR-409-3p was significantly downregulated in BC tissues and cell lines, compared with the corresponding control counterparts. Overexpression of miR-409-3p inhibited BC cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Notably, miR-409-3p induced downregulation of Akt1 protein through binding to its 3′ untranslated region (UTR). Conversely, restoring Akt1 expression rescued the suppressive effects of miR-409-3p. Our data collectively indicate that miR-409-3p functions as a tumor suppressor in BC through downregulating Akt1, supporting the targeting of the novel miR-409-3p/Akt1 axis as a potentially effective therapeutic approach for BC. •miR-409-3p inhibits proliferation, migration and invasion of BC cells.•miR-409-3p suppresses tumor growth in nude mice.•Akt1 is a direct downstream target of miR-409-3p.•Ectopic expression of Akt1 reverses the effects of miR-409-3p on cell proliferation, migration and invasion.