Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein

•RACK1 formed a complex with Annexin A7.•Depletion of RACK1 inhibited the proliferation, migration and invasion.•RACK1 RNAi abolished RACK1-Annexin A7 interaction.•RACK1-Annexin A7 may play a role in regulating the metastatic potentials. We report for the first time that Guanine nucleotide-binding protein subunit beta-2-like 1 (RACK1) formed a complex with Annexin A7. Hca-F and Hca-P are a pair of syngeneic mouse hepatocarcinoma cell lines established and maintained in our laboratory. Our previous study showed that both Annexin A7 and RACK1 were expressed higher in Hca-F (lymph node metastasis >70%) than Hca-P (lymph node metastasis