Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray
► Protein microarray shows the differential expression of 27 proteins induced by RFR. ► RPA32 related to DNA repair is down-regulated in Western blot. ► p73 related to cell genome stability and apoptosis is up-regulated in Western blot. In the present study, the protein microarray was used to invest...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2013-03, Vol.433 (1), p.36-39 |
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| creator | Zhijian, Chen Xiaoxue, Li Wei, Zheng Yezhen, Lu Jianlin, Lou Deqiang, Lu Shijie, Chen Lifen, Jin Jiliang, He |
| description | ► Protein microarray shows the differential expression of 27 proteins induced by RFR. ► RPA32 related to DNA repair is down-regulated in Western blot. ► p73 related to cell genome stability and apoptosis is up-regulated in Western blot.
In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0W/kg for 24h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P |
| doi_str_mv | 10.1016/j.bbrc.2013.02.071 |
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In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0W/kg for 24h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P<0.05). The results validated with Western blot assay indicated that the expression of RPA32 was significantly down-regulated (P<0.05) while the expression of p73 was significantly up-regulated in RFR exposure group (P<0.05). Because of the crucial roles of those proteins in DNA repair and cell apoptosis, the results of present investigation may explain the biological effects of RFR on DNA damage/repair and cell apoptosis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2013.02.071</identifier><identifier>PMID: 23454122</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ABSORPTION ; APOPTOSIS ; Apoptosis - radiation effects ; BIOLOGICAL EFFECTS ; CELL CYCLE ; Cell Cycle - radiation effects ; Cell Line ; CELL PROLIFERATION ; Cell Proliferation - radiation effects ; Cell Transformation, Neoplastic - radiation effects ; DNA DAMAGES ; DNA REPAIR ; DNA Repair - radiation effects ; DNA-Binding Proteins - metabolism ; DNA-Binding Proteins - radiation effects ; Down-Regulation - radiation effects ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit - radiation effects ; Nuclear Proteins - metabolism ; Nuclear Proteins - radiation effects ; p73 ; Precursor Cells, B-Lymphoid - metabolism ; Precursor Cells, B-Lymphoid - radiation effects ; Protein Array Analysis ; Protein microarray ; PROTEINS ; Proteins - metabolism ; Proteins - radiation effects ; Radio Waves - adverse effects ; Radiofrequency radiation ; RADIOWAVE RADIATION ; Replication Protein A ; Replication Protein A - metabolism ; Replication Protein A - radiation effects ; Tumor Protein p73 ; Tumor Suppressor Proteins - metabolism ; Tumor Suppressor Proteins - radiation effects ; Up-Regulation - radiation effects</subject><ispartof>Biochemical and biophysical research communications, 2013-03, Vol.433 (1), p.36-39</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-b96d6333b9c592c5feab4c0160a059cb8bfe1e5bc11c55f14fa573ff762fb4403</citedby><cites>FETCH-LOGICAL-c384t-b96d6333b9c592c5feab4c0160a059cb8bfe1e5bc11c55f14fa573ff762fb4403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X13003446$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23454122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22239526$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhijian, Chen</creatorcontrib><creatorcontrib>Xiaoxue, Li</creatorcontrib><creatorcontrib>Wei, Zheng</creatorcontrib><creatorcontrib>Yezhen, Lu</creatorcontrib><creatorcontrib>Jianlin, Lou</creatorcontrib><creatorcontrib>Deqiang, Lu</creatorcontrib><creatorcontrib>Shijie, Chen</creatorcontrib><creatorcontrib>Lifen, Jin</creatorcontrib><creatorcontrib>Jiliang, He</creatorcontrib><title>Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► Protein microarray shows the differential expression of 27 proteins induced by RFR. ► RPA32 related to DNA repair is down-regulated in Western blot. ► p73 related to cell genome stability and apoptosis is up-regulated in Western blot.
In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0W/kg for 24h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P<0.05). The results validated with Western blot assay indicated that the expression of RPA32 was significantly down-regulated (P<0.05) while the expression of p73 was significantly up-regulated in RFR exposure group (P<0.05). Because of the crucial roles of those proteins in DNA repair and cell apoptosis, the results of present investigation may explain the biological effects of RFR on DNA damage/repair and cell apoptosis.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ABSORPTION</subject><subject>APOPTOSIS</subject><subject>Apoptosis - radiation effects</subject><subject>BIOLOGICAL EFFECTS</subject><subject>CELL CYCLE</subject><subject>Cell Cycle - radiation effects</subject><subject>Cell Line</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - radiation effects</subject><subject>Cell Transformation, Neoplastic - radiation effects</subject><subject>DNA DAMAGES</subject><subject>DNA REPAIR</subject><subject>DNA Repair - radiation effects</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Binding Proteins - radiation effects</subject><subject>Down-Regulation - radiation effects</subject><subject>Humans</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - radiation effects</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear Proteins - radiation effects</subject><subject>p73</subject><subject>Precursor Cells, B-Lymphoid - metabolism</subject><subject>Precursor Cells, B-Lymphoid - radiation effects</subject><subject>Protein Array Analysis</subject><subject>Protein microarray</subject><subject>PROTEINS</subject><subject>Proteins - metabolism</subject><subject>Proteins - radiation effects</subject><subject>Radio Waves - adverse effects</subject><subject>Radiofrequency radiation</subject><subject>RADIOWAVE RADIATION</subject><subject>Replication Protein A</subject><subject>Replication Protein A - metabolism</subject><subject>Replication Protein A - radiation effects</subject><subject>Tumor Protein p73</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Tumor Suppressor Proteins - radiation effects</subject><subject>Up-Regulation - radiation effects</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1DAUtRAVHQo_wAJZYtMuEvxKZiKxgYpOKxUhtSCxs2znmniUxKntAcJP8Ms4Hdolq6srnce95yD0ipKSElq_3ZVaB1MyQnlJWEnW9AlaUdKQglEinqIVIaQuWEO_HaPnMe4IoVTUzTN0zLioBGVshf7cpn07u_E7Th3gKfgEbsTwawoQo_Mjzlu3H9SIP-B-HqbO617F5F2LDfR9XKA-QouTx7TcFNvL3_h0e_vpDAfVOm8D3O1hNPP9qtKieHpzcXOGf7rUPfoNzgSvQlDzC3RkVR_h5b95gr5efPxyfllcf95enb-_LgzfiFTopm5rzrluTNUwU1lQWpgcClGkaozeaAsUKm0oNVVlqbCqWnNr1zWzWgjCT9Cbg66PycloXALTGT-OYJJkjPGmYnVGsQMqnxdjACun4AYVZkmJXDqQO7l0IJcOJGEyd5BJrw-kaa8HaB8pD6FnwLsDAPKDPxyExT-HBK0Li33r3f_0_wImcZlg</recordid><startdate>20130329</startdate><enddate>20130329</enddate><creator>Zhijian, Chen</creator><creator>Xiaoxue, Li</creator><creator>Wei, Zheng</creator><creator>Yezhen, Lu</creator><creator>Jianlin, Lou</creator><creator>Deqiang, Lu</creator><creator>Shijie, Chen</creator><creator>Lifen, Jin</creator><creator>Jiliang, He</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>20130329</creationdate><title>Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray</title><author>Zhijian, Chen ; Xiaoxue, Li ; Wei, Zheng ; Yezhen, Lu ; Jianlin, Lou ; Deqiang, Lu ; Shijie, Chen ; Lifen, Jin ; Jiliang, He</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-b96d6333b9c592c5feab4c0160a059cb8bfe1e5bc11c55f14fa573ff762fb4403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ABSORPTION</topic><topic>APOPTOSIS</topic><topic>Apoptosis - radiation effects</topic><topic>BIOLOGICAL EFFECTS</topic><topic>CELL CYCLE</topic><topic>Cell Cycle - radiation effects</topic><topic>Cell Line</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - radiation effects</topic><topic>Cell Transformation, Neoplastic - radiation effects</topic><topic>DNA DAMAGES</topic><topic>DNA REPAIR</topic><topic>DNA Repair - radiation effects</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Binding Proteins - radiation effects</topic><topic>Down-Regulation - radiation effects</topic><topic>Humans</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - radiation effects</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nuclear Proteins - radiation effects</topic><topic>p73</topic><topic>Precursor Cells, B-Lymphoid - metabolism</topic><topic>Precursor Cells, B-Lymphoid - radiation effects</topic><topic>Protein Array Analysis</topic><topic>Protein microarray</topic><topic>PROTEINS</topic><topic>Proteins - metabolism</topic><topic>Proteins - radiation effects</topic><topic>Radio Waves - adverse effects</topic><topic>Radiofrequency radiation</topic><topic>RADIOWAVE RADIATION</topic><topic>Replication Protein A</topic><topic>Replication Protein A - metabolism</topic><topic>Replication Protein A - radiation effects</topic><topic>Tumor Protein p73</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Tumor Suppressor Proteins - radiation effects</topic><topic>Up-Regulation - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhijian, Chen</creatorcontrib><creatorcontrib>Xiaoxue, Li</creatorcontrib><creatorcontrib>Wei, Zheng</creatorcontrib><creatorcontrib>Yezhen, Lu</creatorcontrib><creatorcontrib>Jianlin, Lou</creatorcontrib><creatorcontrib>Deqiang, Lu</creatorcontrib><creatorcontrib>Shijie, Chen</creatorcontrib><creatorcontrib>Lifen, Jin</creatorcontrib><creatorcontrib>Jiliang, He</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhijian, Chen</au><au>Xiaoxue, Li</au><au>Wei, Zheng</au><au>Yezhen, Lu</au><au>Jianlin, Lou</au><au>Deqiang, Lu</au><au>Shijie, Chen</au><au>Lifen, Jin</au><au>Jiliang, He</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2013-03-29</date><risdate>2013</risdate><volume>433</volume><issue>1</issue><spage>36</spage><epage>39</epage><pages>36-39</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► Protein microarray shows the differential expression of 27 proteins induced by RFR. ► RPA32 related to DNA repair is down-regulated in Western blot. ► p73 related to cell genome stability and apoptosis is up-regulated in Western blot.
In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0W/kg for 24h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P<0.05). The results validated with Western blot assay indicated that the expression of RPA32 was significantly down-regulated (P<0.05) while the expression of p73 was significantly up-regulated in RFR exposure group (P<0.05). Because of the crucial roles of those proteins in DNA repair and cell apoptosis, the results of present investigation may explain the biological effects of RFR on DNA damage/repair and cell apoptosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23454122</pmid><doi>10.1016/j.bbrc.2013.02.071</doi><tpages>4</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES ABSORPTION APOPTOSIS Apoptosis - radiation effects BIOLOGICAL EFFECTS CELL CYCLE Cell Cycle - radiation effects Cell Line CELL PROLIFERATION Cell Proliferation - radiation effects Cell Transformation, Neoplastic - radiation effects DNA DAMAGES DNA REPAIR DNA Repair - radiation effects DNA-Binding Proteins - metabolism DNA-Binding Proteins - radiation effects Down-Regulation - radiation effects Humans Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Hypoxia-Inducible Factor 1, alpha Subunit - radiation effects Nuclear Proteins - metabolism Nuclear Proteins - radiation effects p73 Precursor Cells, B-Lymphoid - metabolism Precursor Cells, B-Lymphoid - radiation effects Protein Array Analysis Protein microarray PROTEINS Proteins - metabolism Proteins - radiation effects Radio Waves - adverse effects Radiofrequency radiation RADIOWAVE RADIATION Replication Protein A Replication Protein A - metabolism Replication Protein A - radiation effects Tumor Protein p73 Tumor Suppressor Proteins - metabolism Tumor Suppressor Proteins - radiation effects Up-Regulation - radiation effects |
| title | Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray |
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