Targeted disruption of the mouse Lipoma Preferred Partner gene
LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2009-02, Vol.379 (2), p.368-373 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Vervenne, Hilke B.V.K. Crombez, Koen R.M.O. Delvaux, Els L. Janssens, Veerle Van de Ven, Wim J.M. Petit, Marleen M.R. |
| description | LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/−×Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter. |
| doi_str_mv | 10.1016/j.bbrc.2008.12.074 |
| format | Article |
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We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/−×Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2008.12.074</identifier><identifier>PMID: 19111675</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Aberrant pregnancy ; Animals ; Cell Nucleus - metabolism ; Cytoskeletal Proteins - genetics ; Cytoskeletal Proteins - metabolism ; Cytoskeletal Proteins - physiology ; Embryo Loss - genetics ; Embryonic Development - genetics ; Female ; Female lethality ; FEMALES ; FERTILITY ; Fertility - genetics ; FIBROBLASTS ; Fibroblasts - metabolism ; Gene Targeting ; GENES ; Knockout ; LIM Domain Proteins ; Lipoma Preferred Partner ; LPP ; Male ; MALES ; MICE ; Mice, Knockout ; Migration ; ONTOGENESIS ; PREGNANCY ; Promoter Regions, Genetic ; PROMOTERS ; Protein Biosynthesis - genetics ; Reproduction - genetics ; Testis - metabolism ; Transcription, Genetic ; TRIP6 ; ZRP-1 ; Zyxin</subject><ispartof>Biochemical and biophysical research communications, 2009-02, Vol.379 (2), p.368-373</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-104ab38aa028b9aae83bd671918ee29695caee068a1e388dada9b99359c63f7e3</citedby><cites>FETCH-LOGICAL-c413t-104ab38aa028b9aae83bd671918ee29695caee068a1e388dada9b99359c63f7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2008.12.074$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19111675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/21255861$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Vervenne, Hilke B.V.K.</creatorcontrib><creatorcontrib>Crombez, Koen R.M.O.</creatorcontrib><creatorcontrib>Delvaux, Els L.</creatorcontrib><creatorcontrib>Janssens, Veerle</creatorcontrib><creatorcontrib>Van de Ven, Wim J.M.</creatorcontrib><creatorcontrib>Petit, Marleen M.R.</creatorcontrib><title>Targeted disruption of the mouse Lipoma Preferred Partner gene</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/−×Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Aberrant pregnancy</subject><subject>Animals</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Cytoskeletal Proteins - physiology</subject><subject>Embryo Loss - genetics</subject><subject>Embryonic Development - genetics</subject><subject>Female</subject><subject>Female lethality</subject><subject>FEMALES</subject><subject>FERTILITY</subject><subject>Fertility - genetics</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Targeting</subject><subject>GENES</subject><subject>Knockout</subject><subject>LIM Domain Proteins</subject><subject>Lipoma Preferred Partner</subject><subject>LPP</subject><subject>Male</subject><subject>MALES</subject><subject>MICE</subject><subject>Mice, Knockout</subject><subject>Migration</subject><subject>ONTOGENESIS</subject><subject>PREGNANCY</subject><subject>Promoter Regions, Genetic</subject><subject>PROMOTERS</subject><subject>Protein Biosynthesis - genetics</subject><subject>Reproduction - genetics</subject><subject>Testis - metabolism</subject><subject>Transcription, Genetic</subject><subject>TRIP6</subject><subject>ZRP-1</subject><subject>Zyxin</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctKAzEUhoMoWi8v4EIGBHcz5mRm0gREkOINCrqo4C5kMqc1pTOpSSr49mZowZ2uzuY7P_-FkHOgBVDg18uiabwpGKWiAFbQcbVHRkAlzRnQap-MKKU8ZxLej8hxCEtKASouD8kRSADg43pEbmfaLzBim7U2-M06Wtdnbp7FD8w6twmYTe3adTp79ThH7xP4qn3s0WcL7PGUHMz1KuDZ7p6Qt4f72eQpn748Pk_uprmpoIx5sqObUmhNmWik1ijKpuXjZEMgMsllbTQi5UIDlkK0utWykbKspeHlfIzlCbnc6roQrQrGRjQfxvU9mqgYsLoWHBJ1taXW3n1uMETV2WBwtdI9piyKc1GVjFf_giz5rCoYFNkWNN6FkCpQa2877b8VUDWMoJZqGEENIyhgKo2Qni526pumw_b3Zdd6Am62AKbKviz6IRH2Blvrh0Cts3_p_wAey5bv</recordid><startdate>20090206</startdate><enddate>20090206</enddate><creator>Vervenne, Hilke B.V.K.</creator><creator>Crombez, Koen R.M.O.</creator><creator>Delvaux, Els L.</creator><creator>Janssens, Veerle</creator><creator>Van de Ven, Wim J.M.</creator><creator>Petit, Marleen M.R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20090206</creationdate><title>Targeted disruption of the mouse Lipoma Preferred Partner gene</title><author>Vervenne, Hilke B.V.K. ; Crombez, Koen R.M.O. ; Delvaux, Els L. ; Janssens, Veerle ; Van de Ven, Wim J.M. ; Petit, Marleen M.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-104ab38aa028b9aae83bd671918ee29695caee068a1e388dada9b99359c63f7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Aberrant pregnancy</topic><topic>Animals</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Cytoskeletal Proteins - physiology</topic><topic>Embryo Loss - genetics</topic><topic>Embryonic Development - genetics</topic><topic>Female</topic><topic>Female lethality</topic><topic>FEMALES</topic><topic>FERTILITY</topic><topic>Fertility - genetics</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Targeting</topic><topic>GENES</topic><topic>Knockout</topic><topic>LIM Domain Proteins</topic><topic>Lipoma Preferred Partner</topic><topic>LPP</topic><topic>Male</topic><topic>MALES</topic><topic>MICE</topic><topic>Mice, Knockout</topic><topic>Migration</topic><topic>ONTOGENESIS</topic><topic>PREGNANCY</topic><topic>Promoter Regions, Genetic</topic><topic>PROMOTERS</topic><topic>Protein Biosynthesis - genetics</topic><topic>Reproduction - genetics</topic><topic>Testis - metabolism</topic><topic>Transcription, Genetic</topic><topic>TRIP6</topic><topic>ZRP-1</topic><topic>Zyxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vervenne, Hilke B.V.K.</creatorcontrib><creatorcontrib>Crombez, Koen R.M.O.</creatorcontrib><creatorcontrib>Delvaux, Els L.</creatorcontrib><creatorcontrib>Janssens, Veerle</creatorcontrib><creatorcontrib>Van de Ven, Wim J.M.</creatorcontrib><creatorcontrib>Petit, Marleen M.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vervenne, Hilke B.V.K.</au><au>Crombez, Koen R.M.O.</au><au>Delvaux, Els L.</au><au>Janssens, Veerle</au><au>Van de Ven, Wim J.M.</au><au>Petit, Marleen M.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted disruption of the mouse Lipoma Preferred Partner gene</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2009-02-06</date><risdate>2009</risdate><volume>379</volume><issue>2</issue><spage>368</spage><epage>373</epage><pages>368-373</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/−×Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19111675</pmid><doi>10.1016/j.bbrc.2008.12.074</doi><tpages>6</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Aberrant pregnancy Animals Cell Nucleus - metabolism Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Cytoskeletal Proteins - physiology Embryo Loss - genetics Embryonic Development - genetics Female Female lethality FEMALES FERTILITY Fertility - genetics FIBROBLASTS Fibroblasts - metabolism Gene Targeting GENES Knockout LIM Domain Proteins Lipoma Preferred Partner LPP Male MALES MICE Mice, Knockout Migration ONTOGENESIS PREGNANCY Promoter Regions, Genetic PROMOTERS Protein Biosynthesis - genetics Reproduction - genetics Testis - metabolism Transcription, Genetic TRIP6 ZRP-1 Zyxin |
| title | Targeted disruption of the mouse Lipoma Preferred Partner gene |
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