Targeted disruption of the mouse Lipoma Preferred Partner gene
LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance...
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Veröffentlicht in: | Biochemical and biophysical research communications 2009-02, Vol.379 (2), p.368-373 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp−/− females. Fertility of Lpp−/− males was proven to be normal, however, females from Lpp−/−×Lpp−/− crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp−/− mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp−/− mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2008.12.074 |