Microinjection of recombinant O-GlcNAc transferase potentiates Xenopus oocytes M-phase entry
In order to understand the importance of the cytosolic and nuclear-specific O-linked N-acetylglucosaminylation ( O-GlcNAc) on cell cycle regulation, we recently reported that inhibition of O-GlcNAc transferase (OGT) delayed or blocked Xenopus laevis oocyte germinal vesicle breakdown (GVBD). Here, we...
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Veröffentlicht in: | Biochemical and biophysical research communications 2008-05, Vol.369 (2), p.539-546 |
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Sprache: | eng |
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Zusammenfassung: | In order to understand the importance of the cytosolic and nuclear-specific
O-linked
N-acetylglucosaminylation (
O-GlcNAc) on cell cycle regulation, we recently reported that inhibition of
O-GlcNAc transferase (OGT) delayed or blocked
Xenopus laevis oocyte germinal vesicle breakdown (GVBD). Here, we show that increased levels of the long OGT isoform (ncOGT) accelerate
X. laevis oocyte GVBD. A N-terminally truncated isoform (sOGT) with a similar
in vitro catalytic activity towards a synthetic CKII-derived peptide had no effect, illustrating the important role played by the N-terminal tetratrico-peptide repeats. ncOGT microinjection in the oocytes increases both the speed and extent of
O-GlcNAc addition, leads to a quicker activation of the MPF and MAPK pathways and finally results in a faster GVBD. Microinjection of anti-OGT antibodies leads to a delay of the GVBD kinetics. Our results hence demonstrate that OGT is a key molecule for the timely progression of the cell cycle. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2008.02.063 |