Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence

Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synch...

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Veröffentlicht in:Plant physiology and biochemistry 2014-11, Vol.84 (C), p.224-232
Hauptverfasser: Lu, Lingli, Liao, Xingcheng, Labavitch, John, Yang, Xiaoe, Nelson, Erik, Du, Yonghua, Brown, Patrick H., Tian, Shengke
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container_issue C
container_start_page 224
container_title Plant physiology and biochemistry
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creator Lu, Lingli
Liao, Xingcheng
Labavitch, John
Yang, Xiaoe
Nelson, Erik
Du, Yonghua
Brown, Patrick H.
Tian, Shengke
description Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii. •We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.
doi_str_mv 10.1016/j.plaphy.2014.10.004
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Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii. •We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2014.10.004</identifier><identifier>PMID: 25306525</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Biological and medical sciences ; Cellular distribution ; Fluorescence ; Fundamental and applied biological sciences. 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Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii. •We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</description><subject>Biological and medical sciences</subject><subject>Cellular distribution</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hyperaccumulation</subject><subject>Plant physiology and development</subject><subject>Sedum - metabolism</subject><subject>Sedum alfredii</subject><subject>Speciation</subject><subject>Spectrometry, X-Ray Emission - methods</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuK1EAUhoMoTjv6BiKFILhJe-qWrtoIw-ANBlyMgrgJlcoJXU1SFasqYnwL39hk0urO1YGf79z-vyieUthToNWr037szXic9wyoWKQ9gLhX7Kg68JJVGu4XO9CKllowdVE8SukEAEwc-MPigkkOlWRyV_y6HdE6k13wxPiW9MGa3v3chNCRr544T_IRyXEeMRprp2HqTQ6R3GI7DcT0XcTWOdLMBH9k9C225EsZzUxMk0Ic7yZ1ziNJOU42TxHvNg3OxlBuZNdPIWKy6C0-Lh50pk_45Fwvi89v33y6fl_efHz34frqprSikrnkqCrOpGpsoxp5UCArtFVnWgEgqeg4AnTWmIYJzUEB74xGq_AgeKuZEfyyeL7NDSm7OlmX0R5t8B5trqmUFZMr9HKDxhi-TZhyPbjlzL43HsOUaloxrQ-qAlhQsaHLWylF7OoxusHEuaZQr4nVp3pLrF4TW9UlsaXt2XnD1AzY_m36E9ECvDgDJtnVbeOtS_84pTRozhfu9cbhYtp3h3H9aTW0dXF9qQ3u_5f8BronuF4</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Lu, Lingli</creator><creator>Liao, Xingcheng</creator><creator>Labavitch, John</creator><creator>Yang, Xiaoe</creator><creator>Nelson, Erik</creator><creator>Du, Yonghua</creator><creator>Brown, Patrick H.</creator><creator>Tian, Shengke</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20141101</creationdate><title>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</title><author>Lu, Lingli ; Liao, Xingcheng ; Labavitch, John ; Yang, Xiaoe ; Nelson, Erik ; Du, Yonghua ; Brown, Patrick H. ; Tian, Shengke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-3e863258bcb8b578056ec6fad400514f3e00fcaab24930803fa9ec8e743d92a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Cellular distribution</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hyperaccumulation</topic><topic>Plant physiology and development</topic><topic>Sedum - metabolism</topic><topic>Sedum alfredii</topic><topic>Speciation</topic><topic>Spectrometry, X-Ray Emission - methods</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Lingli</creatorcontrib><creatorcontrib>Liao, Xingcheng</creatorcontrib><creatorcontrib>Labavitch, John</creatorcontrib><creatorcontrib>Yang, Xiaoe</creatorcontrib><creatorcontrib>Nelson, Erik</creatorcontrib><creatorcontrib>Du, Yonghua</creatorcontrib><creatorcontrib>Brown, Patrick H.</creatorcontrib><creatorcontrib>Tian, Shengke</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Lingli</au><au>Liao, Xingcheng</au><au>Labavitch, John</au><au>Yang, Xiaoe</au><au>Nelson, Erik</au><au>Du, Yonghua</au><au>Brown, Patrick H.</au><au>Tian, Shengke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>84</volume><issue>C</issue><spage>224</spage><epage>232</epage><pages>224-232</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii. •We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>25306525</pmid><doi>10.1016/j.plaphy.2014.10.004</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Biological and medical sciences
Cellular distribution
Fluorescence
Fundamental and applied biological sciences. Psychology
Hyperaccumulation
Plant physiology and development
Sedum - metabolism
Sedum alfredii
Speciation
Spectrometry, X-Ray Emission - methods
Zinc
Zinc - metabolism
title Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence
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