Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence
Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synch...
Gespeichert in:
Veröffentlicht in: | Plant physiology and biochemistry 2014-11, Vol.84 (C), p.224-232 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 232 |
---|---|
container_issue | C |
container_start_page | 224 |
container_title | Plant physiology and biochemistry |
container_volume | 84 |
creator | Lu, Lingli Liao, Xingcheng Labavitch, John Yang, Xiaoe Nelson, Erik Du, Yonghua Brown, Patrick H. Tian, Shengke |
description | Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii.
•We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii. |
doi_str_mv | 10.1016/j.plaphy.2014.10.004 |
format | Article |
fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_1556254</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0981942814003088</els_id><sourcerecordid>1629978600</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-3e863258bcb8b578056ec6fad400514f3e00fcaab24930803fa9ec8e743d92a43</originalsourceid><addsrcrecordid>eNp9kcuK1EAUhoMoTjv6BiKFILhJe-qWrtoIw-ANBlyMgrgJlcoJXU1SFasqYnwL39hk0urO1YGf79z-vyieUthToNWr037szXic9wyoWKQ9gLhX7Kg68JJVGu4XO9CKllowdVE8SukEAEwc-MPigkkOlWRyV_y6HdE6k13wxPiW9MGa3v3chNCRr544T_IRyXEeMRprp2HqTQ6R3GI7DcT0XcTWOdLMBH9k9C225EsZzUxMk0Ic7yZ1ziNJOU42TxHvNg3OxlBuZNdPIWKy6C0-Lh50pk_45Fwvi89v33y6fl_efHz34frqprSikrnkqCrOpGpsoxp5UCArtFVnWgEgqeg4AnTWmIYJzUEB74xGq_AgeKuZEfyyeL7NDSm7OlmX0R5t8B5trqmUFZMr9HKDxhi-TZhyPbjlzL43HsOUaloxrQ-qAlhQsaHLWylF7OoxusHEuaZQr4nVp3pLrF4TW9UlsaXt2XnD1AzY_m36E9ECvDgDJtnVbeOtS_84pTRozhfu9cbhYtp3h3H9aTW0dXF9qQ3u_5f8BronuF4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1629978600</pqid></control><display><type>article</type><title>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Lu, Lingli ; Liao, Xingcheng ; Labavitch, John ; Yang, Xiaoe ; Nelson, Erik ; Du, Yonghua ; Brown, Patrick H. ; Tian, Shengke</creator><creatorcontrib>Lu, Lingli ; Liao, Xingcheng ; Labavitch, John ; Yang, Xiaoe ; Nelson, Erik ; Du, Yonghua ; Brown, Patrick H. ; Tian, Shengke</creatorcontrib><description>Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii.
•We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2014.10.004</identifier><identifier>PMID: 25306525</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Biological and medical sciences ; Cellular distribution ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Hyperaccumulation ; Plant physiology and development ; Sedum - metabolism ; Sedum alfredii ; Speciation ; Spectrometry, X-Ray Emission - methods ; Zinc ; Zinc - metabolism</subject><ispartof>Plant physiology and biochemistry, 2014-11, Vol.84 (C), p.224-232</ispartof><rights>2014 Elsevier Masson SAS</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-3e863258bcb8b578056ec6fad400514f3e00fcaab24930803fa9ec8e743d92a43</citedby><cites>FETCH-LOGICAL-c465t-3e863258bcb8b578056ec6fad400514f3e00fcaab24930803fa9ec8e743d92a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plaphy.2014.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28890933$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25306525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1556254$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Lingli</creatorcontrib><creatorcontrib>Liao, Xingcheng</creatorcontrib><creatorcontrib>Labavitch, John</creatorcontrib><creatorcontrib>Yang, Xiaoe</creatorcontrib><creatorcontrib>Nelson, Erik</creatorcontrib><creatorcontrib>Du, Yonghua</creatorcontrib><creatorcontrib>Brown, Patrick H.</creatorcontrib><creatorcontrib>Tian, Shengke</creatorcontrib><title>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii.
•We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</description><subject>Biological and medical sciences</subject><subject>Cellular distribution</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hyperaccumulation</subject><subject>Plant physiology and development</subject><subject>Sedum - metabolism</subject><subject>Sedum alfredii</subject><subject>Speciation</subject><subject>Spectrometry, X-Ray Emission - methods</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuK1EAUhoMoTjv6BiKFILhJe-qWrtoIw-ANBlyMgrgJlcoJXU1SFasqYnwL39hk0urO1YGf79z-vyieUthToNWr037szXic9wyoWKQ9gLhX7Kg68JJVGu4XO9CKllowdVE8SukEAEwc-MPigkkOlWRyV_y6HdE6k13wxPiW9MGa3v3chNCRr544T_IRyXEeMRprp2HqTQ6R3GI7DcT0XcTWOdLMBH9k9C225EsZzUxMk0Ic7yZ1ziNJOU42TxHvNg3OxlBuZNdPIWKy6C0-Lh50pk_45Fwvi89v33y6fl_efHz34frqprSikrnkqCrOpGpsoxp5UCArtFVnWgEgqeg4AnTWmIYJzUEB74xGq_AgeKuZEfyyeL7NDSm7OlmX0R5t8B5trqmUFZMr9HKDxhi-TZhyPbjlzL43HsOUaloxrQ-qAlhQsaHLWylF7OoxusHEuaZQr4nVp3pLrF4TW9UlsaXt2XnD1AzY_m36E9ECvDgDJtnVbeOtS_84pTRozhfu9cbhYtp3h3H9aTW0dXF9qQ3u_5f8BronuF4</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Lu, Lingli</creator><creator>Liao, Xingcheng</creator><creator>Labavitch, John</creator><creator>Yang, Xiaoe</creator><creator>Nelson, Erik</creator><creator>Du, Yonghua</creator><creator>Brown, Patrick H.</creator><creator>Tian, Shengke</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20141101</creationdate><title>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</title><author>Lu, Lingli ; Liao, Xingcheng ; Labavitch, John ; Yang, Xiaoe ; Nelson, Erik ; Du, Yonghua ; Brown, Patrick H. ; Tian, Shengke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-3e863258bcb8b578056ec6fad400514f3e00fcaab24930803fa9ec8e743d92a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Cellular distribution</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hyperaccumulation</topic><topic>Plant physiology and development</topic><topic>Sedum - metabolism</topic><topic>Sedum alfredii</topic><topic>Speciation</topic><topic>Spectrometry, X-Ray Emission - methods</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Lingli</creatorcontrib><creatorcontrib>Liao, Xingcheng</creatorcontrib><creatorcontrib>Labavitch, John</creatorcontrib><creatorcontrib>Yang, Xiaoe</creatorcontrib><creatorcontrib>Nelson, Erik</creatorcontrib><creatorcontrib>Du, Yonghua</creatorcontrib><creatorcontrib>Brown, Patrick H.</creatorcontrib><creatorcontrib>Tian, Shengke</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Lingli</au><au>Liao, Xingcheng</au><au>Labavitch, John</au><au>Yang, Xiaoe</au><au>Nelson, Erik</au><au>Du, Yonghua</au><au>Brown, Patrick H.</au><au>Tian, Shengke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>84</volume><issue>C</issue><spage>224</spage><epage>232</epage><pages>224-232</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>Differences in metal homeostasis among related plant species can give important information of metal hyperaccumulation mechanisms. Speciation and distribution of Zn were investigated in a hyperaccumulating population of Sedum alfredii by using extended X-ray absorption fine structure and micro-synchrotron X-ray fluorescence (μ-XRF), respectively. The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage in leaves and stems, and variations in the Zn speciation was noted in different tissues. The dominant chemical form of Zn in leaves was most probably a complex with malate, the most prevalent organic acid in S. alfredii leaves. In stems, Zn was mainly associated with malate and cell walls, while Zn–citrate and Zn–cell wall complexes dominated in the roots. Two-dimensional μ-XRF images revealed age-dependent differences in Zn localization in S. alfredii stems and leaves. In old leaves of S. alfredii, Zn was high in the midrib, margin regions and the petiole, whereas distribution of Zn was essentially uniform in young leaves. Zinc was preferentially sequestered by cells near vascular bundles in young stems, but was highly localized to vascular bundles and the outer cortex layer of old stems. The results suggest that tissue- and age-dependent variations of Zn speciation and distribution occurred in the hyperaccumulator S. alfredii, with most of the Zn complexed with malate in the leaves, but a shift to cell wall– and citric acid–Zn complexes during transportation and storage in stems and roots. This implies that biotransformation in Zn complexation occurred during transportation and storage processes in the plants of S. alfredii.
•We investigate speciation and distribution of Zn in a hyperaccumulator Sedum alfredii by EXAFS and μ-XRF.•The hyperaccumulator uses complexation with oxygen donor ligands for Zn storage.•The dominant chemical form of Zn in leaves of S. alfredii was a complex with malate.•Tissue- and age-dependent variations of Zn speciation and distribution occurred in S. alfredii.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>25306525</pmid><doi>10.1016/j.plaphy.2014.10.004</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0981-9428 |
ispartof | Plant physiology and biochemistry, 2014-11, Vol.84 (C), p.224-232 |
issn | 0981-9428 1873-2690 |
language | eng |
recordid | cdi_osti_scitechconnect_1556254 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Biological and medical sciences Cellular distribution Fluorescence Fundamental and applied biological sciences. Psychology Hyperaccumulation Plant physiology and development Sedum - metabolism Sedum alfredii Speciation Spectrometry, X-Ray Emission - methods Zinc Zinc - metabolism |
title | Speciation and localization of Zn in the hyperaccumulator Sedum alfredii by extended X-ray absorption fine structure and micro-X-ray fluorescence |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T16%3A30%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Speciation%20and%20localization%20of%20Zn%20in%20the%20hyperaccumulator%20Sedum%20alfredii%20by%20extended%20X-ray%20absorption%20fine%20structure%20and%20micro-X-ray%20fluorescence&rft.jtitle=Plant%20physiology%20and%20biochemistry&rft.au=Lu,%20Lingli&rft.date=2014-11-01&rft.volume=84&rft.issue=C&rft.spage=224&rft.epage=232&rft.pages=224-232&rft.issn=0981-9428&rft.eissn=1873-2690&rft.coden=PPBIEX&rft_id=info:doi/10.1016/j.plaphy.2014.10.004&rft_dat=%3Cproquest_osti_%3E1629978600%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1629978600&rft_id=info:pmid/25306525&rft_els_id=S0981942814003088&rfr_iscdi=true |