Genome-Wide Sequencing of 41 Rice (Oryza sativa L.) Mutated Lines Reveals Diverse Mutations Induced by Fast-Neutron Irradiation

Fast-neutron (FN) irradiation has been used to create mutagen-ized collections of many plant species (Bolon et al., 2014). FN-induced mutagenesis has clear advantages: it is an efficientmeans of saturating the genome, and it does not involve time-consuming plant transformation or tissue culture. In rice, mostmutant collections, although highly valuable, were generated us-ing either T-DNA insertion or transposon tagging approaches thatoften induce mutations unlinked to the insertion and complicatinganalysis (Wang et al., 2013). Another disadvantage of some ofthese collections is that they were created in rice varieties thatare photoperiod-sensitive and often have long generation times.