Multiple efficacy studies of an adenovirus-vectored foot-and-mouth disease virus serotype A24 subunit vaccine in cattle using homologous challenge

Highlights • FMDV serotype A24 replication deficient adenovirus-vector vaccine was safe after IM dosing. • AdtA24 vaccine was immunogenic; antibody titers to FMDV A24 Cruzeiro were dose dependent. • AdtA24 protected cattle from clinical disease and viremia after FMDV A24 challenge at 7 dpv. • AdtA24 was a DIVA vaccine based on post-vaccination/pre-challenge absence of NSP antibodies. • Non-vaccinated animals co-mingled with vaccinates did not seroconvert to the adenovector.