Molecular modeling of the elastomeric properties of repeating units and building blocks of resilin, a disordered elastic protein

The mechanisms responsible for the properties of disordered elastomeric proteins are not well known. To better understand the relationship between elastomeric behavior and amino acid sequence, we investigated resilin, a disordered rubber-like protein, found in specialized regions of the cuticle of insects. Resilin of Drosophila melanogaster contains Gly-rich repetitive motifs comprised of the amino acids, PSSSYGAPGGGNGGR, which confer elastic properties to resilin. The repetitive motifs of insect resilin can be divided into smaller partially conserved building blocks: PSS, SYGAP, GGGN and GGR. Using molecular dynamics (MD) simulations, we studied the relative roles of SYGAP, and its less common variants SYSAP and TYGAP, on the elastomeric properties of resilin. Results showed that SYGAP adopts a bent structure that is one-half to one-third the end-to-end length of the other motifs having an equal number of amino acids but containing SYSAP or TYGAP substituted for SYGAP. The bent structure of SYGAP forms due to conformational freedom of glycine, and hydrogen bonding within the motif apparently plays a role in maintaining this conformation. These structural features of SYGAP result in higher extensibility compared to other motifs, which may contribute to elastic properties at the macroscopic level. Overall, the results are consistent with a role for the SYGAP building block in the elastomeric properties of these disordered proteins. What we learned from simulating the repetitive motifs of resilin may be applicable to the biology and mechanics of other elastomeric biomaterials, and may provide us the deeper understanding of their unique properties. Fig. Hydrogen bonds in the repeat motif SYGAP during bent conformation. Other than proline, all the other amino acids participate in hydrogen bonding to form the stable bent structure in addition to lack of streric hindrance at glycine (arrow). ▪ •MD simulations were used to characterize stiffness of amino acid sequences.•Repetitive motif sequence and order of amino acid strongly influence chain stiffness.•Certain repeat motifs favor bent structure conformations with low stiffness.•Intramolecular hydrogen bonds may help maintain bent conformation.•The amino acid sequence corresponding to resilin has very low stiffness.