Species differences in unlocking B‐side electron transfer in bacterial reaction centers

The structure of the bacterial photosynthetic reaction center (RC) reveals symmetry‐related electron transfer (ET) pathways, but only one path is used in native RCs. Analogous mutations have been made in two Rhodobacter (R.) species. A glutamic acid at position 133 in the M subunit increases transme...

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Veröffentlicht in:FEBS letters 2016-08, Vol.590 (16), p.2515-2526
Hauptverfasser: Dylla, Nicholas P., Faries, Kaitlyn M., Wyllie, Ryan M., Swenson, Angela M., Hanson, Deborah K., Holten, Dewey, Kirmaier, Christine, Laible, Philip D.
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Sprache:eng
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Zusammenfassung:The structure of the bacterial photosynthetic reaction center (RC) reveals symmetry‐related electron transfer (ET) pathways, but only one path is used in native RCs. Analogous mutations have been made in two Rhodobacter (R.) species. A glutamic acid at position 133 in the M subunit increases transmembrane charge separation via the naturally inactive (B‐side) path through impacts on primary ET in mutant R. sphaeroidesRCs. Prior work showed that the analogous substitution in the R. capsulatusRC also increases B‐side activity, but mainly affects secondary ET. The overall yields of transmembrane ET are similar, but enabled in fundamentally different ways. Streamlined mutagenesis system for generation of R. sphaeroides RC mutants. R. sphaeroides RC with B‐side P+HB− yield of ~60%. High yields of transmembrane B‐side charge separation forming P+QB−.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12264