Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacill...
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| Veröffentlicht in: | Nature methods 2015-11, Vol.13 |
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| container_title | Nature methods |
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| creator | Sierra, Raymond G. Gati, Cornelius Laksmono, Hartawan Dao, E. Han Gul, Sheraz Fuller, Franklin Kern, Jan Chatterjee, Ruchira Ibrahim, Mohamed Brewster, Aaron S. Young, Iris D. Michels-Clark, Tara Aquila, Andrew Liang, Mengning Hunter, Mark S. Koglin, Jason E. Boutet, Sébastien Junco, Elia A. Hayes, Brandon Bogan, Michael J. Hampton, Christina Y. Puglisi, Elisabetta V. Sauter, Nicholas K. Stan, Claudiu A. Zouni, Athina Yano, Junko Yachandra, Vittal K. Soltis, S. Michael Puglisi, Joseph D. DeMirci, Hasan |
| description | We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II ( |
| format | Article |
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| title | Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II |
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