Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacill...

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Veröffentlicht in:Nature methods 2015-11, Vol.13
Hauptverfasser: Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., DeMirci, Hasan
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Sprache:eng
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Zusammenfassung:We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (
ISSN:1548-7091
1548-7105