Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot–Enzyme Biohybrid Complexes
We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent k cat of 1400 h–1. Regeneration of NADPH was coupled to reduction o...
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Veröffentlicht in: | ACS catalysis 2016-04, Vol.6 (4), p.2201-2204 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent k cat of 1400 h–1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5–6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations. |
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ISSN: | 2155-5435 2155-5435 |
DOI: | 10.1021/acscatal.5b02850 |